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. 2010 Sep 10;6(5):513–524. doi: 10.7150/ijbs.6.513

Figure 1.

Figure 1

Cister search results and PCR amplification of ChIP DNA samples from MCF7 and Hs578T cells. A. Cister predicts regulatory regions in DNA sequences by searching for clusters of cis-elements. The red line adjacent to the pink protein coding region corresponds to the putative BP1 binding site located on the positive strand of the human BRCA1 gene and indicates the probability that a regulatory factor binds at this position. B. ChIP assays were conducted with a BP1 antibody and a no antibody control. A 186 base pair PCR fragment was amplified with BP1 immunoprecipitated and input DNA from BP1 overexpressing MCF7 and Hs578T cells, but not with the no antibody control, using primers flanking the putative BP1 binding site. C. Correlation of BP1 mRNA expression with the level of ChIPed BRCA1 genomic DNA analysis. ChIP assays were performed on a series of MCF7 cell lines with different levels of BP1 expression. Various levels of BRCA1 genomic DNA were amplified from the ChIP DNA by SYBR real-time PCR, in correlation with the mRNA expression of BP1 in each cell line.