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. 2010 Sep 10;6(5):513–524. doi: 10.7150/ijbs.6.513

Figure 2.

Figure 2

Electrophoretic mobility shift assay (EMSA). Protein extract isolated from BP1 overexpressing MCF7 cells binds specifically to a 33- nucleotide double-stranded sequence containing the putative BP1 binding site in the first intron of BRCA1. Labeled probe specific for the BP1 binding site was used in the binding reactions with the following additions: Lane 1, free probe; lane 2, protein extract; lane 3, protein extract plus 50-fold molar excess of unlabeled BP1 specific double-stranded probe; lane 4, protein extract plus 100-fold molar excess of unlabeled BP1 specific double-stranded probe; lane 5, protein extract plus 100-fold molar excess of unlabeled scrambled probe.