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Western blot analysis of BP1 and BRCA1 protein levels. Whole-cell extracts from MCF7 vector controls (V1 and V2) and BP1 overexpressors (O1, O2, and O4) were analyzed by Western blotting using antibodies for BP1 or BRCA1 (4A). Protein expression levels were normalized against β-actin. BRCA1 protein expression is decreased by 72-79% in BP1 overexpressors relative to vector controls. Relative BP1 and BRCA1 expression levels are presented based on results from one experiment each (4B.).
