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. Author manuscript; available in PMC: 2011 Aug 20.
Published in final edited form as: ACS Chem Biol. 2010 Aug 20;5(8):787–795. doi: 10.1021/cb100096f

Figure 2.

Figure 2

The first folate-dependent metabolite to be depleted following trimethoprim addition, accounting for the almost immediate halt in growth, is glycine. a) Growth curves for E. coli with trimethoprim addition (red) and without trimethoprim (black) in minimal media. Error bars show ±1 SE of the mean (n = 3). b) Concentration data for four metabolites from trimethoprim-treated E. coli. Data are shown as relative concentration normalized to concentrations at time 0 and optical density. Trimethoprim was added to cells growing in minimal media at time 0. Error bars show ±1 SE of the mean (n ≥ 2 independent experiments).