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. Author manuscript; available in PMC: 2011 Aug 20.
Published in final edited form as: ACS Chem Biol. 2010 Aug 20;5(8):787–795. doi: 10.1021/cb100096f

Figure 4.

Figure 4

Inosine supplementation leads to thymineless death. a) Effect of trimethoprim (Tm) on the growth of E. coli. Trimethoprim was added at time 0, as indicated by the arrow. Data shown is one representative replicate of at least triplicates. b) Influence of various supplements on viability of wildtype E. coli 24 h post trimethoprim addition. Colony forming units (CFU) were counted 24 h post drug treatment and normalized to the CFU count at 0 h (time of drug addition). The x axis represents CFU fold change over 24 h on a log 10 scale. Error bars show ±1 SE of the mean (n ≥ 2 independent experiments). c) Microscopic images of wildtype cells 24 h post trimethoprim treatment under different conditions. Images taken using Zeiss AxioVision 4.5.