Table 3.
P22 image reconstruction statistics
CP type | Micrographs | Particle Images† | Defocus Range(μm) †† | Resolution (Å) *† |
---|---|---|---|---|
WT* | 164 | 11,997 | 0.41 – 3.65 | 9.1 |
F170L | 33 | 1,757 | 0.73 – 2.79 | 13.5 |
F170K | 38 | 4,123 | 0.77 – 2.96 | 10.4 |
F170A | 72 | 4,008 | 0.83 – 3.26 | 10.3 |
The data for the WT reconstruction is from a previous study (Parent et al., 2010)
Number of boxed particle images used to compute each 3D reconstruction
Range of objective lens underfocus settings used to record micrographs
Estimation of resolution based on FSC0.5 criterion (van Heel & Schatz, 2005)