Figure 1.
Charge deconvoluted “singly charged” ESI-mass spectra of A) Negative control in which reduced RNAse A was allowed to refold in the absence of dimedone at room temperature, pH 8, and exposure to air. “X” indicates the absence of a peak at the expected m/z value of dimedone-modified RNAse A; B) Negative control in which native (non-reduced / folded) RNAse A was exposed to refolding conditions in the presence of 50 mM dimedone; C) Reduced and purified RNAse A refolded in the presence of 50 mM dimedone. The calculated MH+ mass of fully reduced RNAse A is 13691.3 and that of fully folded RNAse A is 13683.2.