Figure 3. Effect of fasudil on inflammatory foci:
Representative sections of untreated (A) and fasudil treated (B) (n = 4/group) quadriceps femoris muscle of SJL/J mice sacrificed at 25 weeks of age. Frozen sections of quadriceps femoris were stained with rat anti-mouse F4/80 (Serotec, Oxford, UK) primary antibody. Anti-rat horse-radish peroxidase (DAKO, Carpinteria, CA) was used as a secondary antibody. Three non-overlapping fields of the entire tissue section were imaged (20X) and quantified. Data were graphed (mean ± SEM) and the groups were compared using student's t-test for independent samples, where *p<0.05 by Student's t-test comparison.
