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. Author manuscript; available in PMC: 2011 Mar 16.
Published in final edited form as: Oncogene. 2010 Jun 28;29(37):5193–5203. doi: 10.1038/onc.2010.257

Figure 3.

Figure 3

RNAi of HER3 partially inhibits growth of PI3K mutant cells. (a) IB comparing HER3 and pAKT levels in cells transfected with either control or HER3 siRNA duplexes. Cells were harvested on day 2 and 6 post-transfection. (b) Determination of cell numbers on day 3, 6 and 7. Each bar represents the mean ± SE of six replicates. (c) Crystal violet staining of transfected cells on day 7. (d) HCC-1954; (e) MDA-MB-453; and (f) UACC893 cells were transfected with control or HER3 siRNA duplexes as indicated Materials and Methods. HER3 and pAKT levels were determined by IB (day 4: HCC1954; day 6: MDA-MB-453; day 4: UACC893). Differences in cell number between control and HER3 siRNA-transfected cells on day 4 for HCC1954; day 5 for MDA-MB-453 and day 7 for UACC893 were determined in a Coulter counter.