Skip to main content
. Author manuscript; available in PMC: 2010 Dec 1.
Published in final edited form as: Surgery. 2009 Dec;146(6):1196–1207. doi: 10.1016/j.surg.2009.09.028

Figure 4. Modulation of Heat Shock Protein Expression by D004.

Figure 4

D004 modulates HSP expression in thyroid cancer cells. Anaplastic (DRO) and papillary (NPA) thyroid cancer cells were treated with 1% DMSO (as control) or D004 at concentrations from 0.25 to 3μM for 24 hr. Lysates were prepared and subjected to Western blotting with the indicated antibodies. HSP90α and HSP27 levels increased slightly at 3μM D004 in both cell lines. HSP90β levels decreased with increasing D004 concentration. Both GRP94 and HSP70 levels increased at 1μM and 3μM D004 in both cell-lines. TRAP-1 levels increased in DRO cells more than NPA cells with increasing D004 concentration. Finally, HSF-1 levels decreased in both cell lines in a dose-dependant manner.