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. Author manuscript; available in PMC: 2010 Dec 1.
Published in final edited form as: Surgery. 2009 Dec;146(6):1196–1207. doi: 10.1016/j.surg.2009.09.028

Figure 5. Modulation of HSP90 Client Protein Expression.

Figure 5

D004 modulates HSP90 client proteins in thyroid cancer cells. Anaplastic (DRO) and papillary (NPA) thyroid cancer cells were treated with 1% DMSO (as control), D004 or 17-AAG at concentrations from 0.1 to 3μM for 24 hr. Lysates were prepared and subjected to Western blotting with the indicated antibodies. D004 and 17-AAG depleted total AKT levels in DRO and NPA cells in a dose-dependant manner, although more potently with D004. Phosphorylated ERK and B-raf levels were increased in both DRO and NPA cell lines after 24 hr treatment in a concentration-dependent manner with D004 treatment. Total ERK protein levels did not changed significantly in DRO and NPA cells.