Skip to main content
. Author manuscript; available in PMC: 2011 Sep 24.
Published in final edited form as: J Mol Biol. 2010 Aug 11;402(3):560–577. doi: 10.1016/j.jmb.2010.08.007

Figure 1. The NHR3 domain of AML1-ETO specifically binds PKA (RIIα).

Figure 1

ITC measurements of the binding of Trx-NHR3 constructs to PKA (RIIα). In each panel, the top portion is the raw data and the bottom portion is a plot of the binding corrected for the dilution enthalpy (experimental data – squares; fit to a one-site binding model – line). The average N (stoichiometry) and Kd from two independent experiments (±SD) for each protein are shown in the box. (A) ITC of N-terminal Trx-NHR3 (585-615) construct. (B) ITC of the wild-type Trx-NHR3 (585-654) construct.