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. Author manuscript; available in PMC: 2011 Oct 1.

Published in final edited form as: Placenta. 2010 Aug 14;31(10):886–892. doi: 10.1016/j.placenta.2010.07.012

Thrombin Induces MMP9 and MMP3 in Amnion: Thrombin (0–100 u/ml for 48 h) induced concentration-dependent increases in both the pro-MMP9 (92 kD) and the active MMP9 (78 kD) protein (Panel A); and in both the pro-MMP3 (57 kD) and the active MMP3 (45 kD) protein (Panel B); in the amnion component of treated FM explants. The Western blot shown is representative of three replicate experiments with three different placentas. To confirm the significance of qualitative results, triplicate blots were scanned and subjected to densitometric analysis. Each blot was normalized to the zero-thrombin controls as indicated in Methods. (Data are presented mean ± SD, * p < 0.01 vs. no thrombin control)

Thrombin Induces MMP9 and MMP3 in Amnion: Thrombin (0–100 u/ml for 48 h) induced concentration-dependent increases in both the pro-MMP9 (92 kD) and the active MMP9 (78 kD) protein (Panel A); and in both the pro-MMP3 (57 kD) and the active MMP3 (45 kD) protein (Panel B); in the amnion component of treated FM explants. The Western blot shown is representative of three replicate experiments with three different placentas. To confirm the significance of qualitative results, triplicate blots were scanned and subjected to densitometric analysis. Each blot was normalized to the zero-thrombin controls as indicated in Methods. (Data are presented mean ± SD, * p < 0.01 vs. no thrombin control)