Fig. 5.

B7-H1 protein expression and MEK inhibitors. a Western blot analysis of B7-H1 levels with anti-mouse B7-H1 mAb (clone 179711) in DA1-3b mouse leukemic cells exposed to 500 IU/ml IFN-γ with or without 1-h pre-incubation with 20 μM UO126 (right panel) or 25 μM rapamycin (left panel). b Western blot analysis of B7H1 levels with anti-human B7-H1 mAb (clone 130002) in blast cells from one patient with AML. Cells were exposed to 500 ng/ml Ultrapure LPS for 24 h with or without pre-incubation with 20 μM UO126 or 1 μM AZD6244. c Same as in (b), but in another AML patient and with exposure to 500 IU/ml IFN-γ instead of LPS