Fig. 6.

The impact of TAP inhibition in Invariant Chain-silenced KG-1 blasts on HLA-DR expression. Ii-silenced KG-1 blasts transduced with BNLF2a/GFP or UL49.5/GFP retrovirus were flow cytometrically sorted for GFP⁺ blasts. Subsequently, we determined Ii versus total HLA-DR expression in permeabilized GFP⁺ blasts (left panel) and the resulting expression levels of HLA-DR on intact, 7AAD⁻ blasts (right panel; unfilled histograms) by flow cytometry. The filled histograms represent stainings of the same cells with control IgG