Table 1.
entry | dsDNA | sequences | ΔH (kcal · mol−1) |
ΔS (cal · K−1 · mol−1) |
Tm (°C) |
---|---|---|---|---|---|
1 | (B:A) | 5′-ATG AAC CBG GAA AAC-3′ 3′-TAC TTG GAC CTT TTG-5′ |
n.a. | n.a. | n.a. |
2 | (B:T) | 5′-ATG AAC CBG GAA AAC-3′ 3′-TAC TTG GTC CTT TTG-5′ |
63.0 | 200 | 45 |
3 | (B:G) | 5′-ATG AAC CBG GAA AAC-3′ 3′-TAC TTG GGC CTT TTG-5′ |
47.5 | 147 | 48 |
4 | (B:C) | 5′-ATG AAC CBG GAA AAC-3′ 3′-TAC TTG GCC CTT TTG-5′ |
66.4 | 198 | 46 |
5 | (T:A) | 5′-ATG AAC CTG GAA AAC-3′ 3′-TAC TTG GAC CTT TTG-5′ |
- | - | 55 |
6 | (C:G) | 5′-ATG AAC CCG GAA AAC-3′ 3′-TAC TTG GGC CTT TTG-5′ |
96.8 | 296 | 58 |