FIGURE 4.

PE_PGRS11 induced rescue from oxidative stress: activation of antiapoptotic and inhibition of apoptotic signaling axis. A, induced expression of COX-2 and Bcl2 upon PE_PGRS11 treatment as analyzed by immunoblotting. B, dose-dependent increase in Bcl2 expression upon PGE₂ treatment. C, A549 cells were pretreated with NS-398, and PE_PGRS11-induced Bcl2 expression was analyzed by immunoblotting. D, pretreatment of A549 cells with U0126 and LY294002 leads to abrogation of PE_PGRS11-induced COX-2 expression. E, activation of p38 MAPK upon exposure of cells to H₂O₂. F, PE_PGRS11 abolishes H₂O₂-mediated activation of p38 MAPK. The blots are representative of three independent experiments. p, phospho; Med, medium.