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. 2010 Jul 27;285(40):30419–30426. doi: 10.1074/jbc.M109.094284

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© 2010 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 3. — Restoration of deficient APM promoter activity in HER-2/neu⁺ cells by selective mutations of TFBS. A, tapasin-mut-luc promoters. B and C, TAP1-mut-luc promoters (B) and TAP2-mut-luc promoters (C) and the pGL3/Enhancer vector, which served as a control (data not shown), were transiently cotransfected with the β-gal vector into HER-2/neu⁻ and HER-2/neu⁺ cells 48 h prior to the determination of the luc activity using a reporter gene assay as described under “Experimental Procedures.” The data were normalized to β-gal activity. For comparison, the WT APM promoter activities in HER-2/neu⁻ cells were set to 100%, and the respective APM promoter activities in HER-2/neu⁺ cells were calculated. Error bars in all panels indicate S.D. rel luc activity, relative luc activity.