FIGURE 1.

Histone H3 is not evicted from the core promoter and coding sequence of GAL1 following transcriptional induction in galactose-containing growth medium in the RTT109 deletion mutant strain. A, the PCR primer pairs located at the core promoter and coding sequence of GAL1 are schematically shown. Core, core promoter. B, Rtt109p is required for histone H3 eviction from the GAL1 core promoter. Both the wild type and RTT109 deletion mutant strains were grown in YPR up to an A₆₀₀ of 0.9 and then switched to YPG for transcriptional induction prior to formaldehyde-based in vivo cross-linking. Immunoprecipitations were performed using an anti-H3 antibody (Ab-1791, Abcam) against histone H3. Immunoprecipitated DNA was analyzed by PCR, using the primer pair targeted to the core promoter of GAL1. C, Rtt109p is required for histone H3 eviction from the GAL1 coding sequence. Immunoprecipitated DNA was analyzed by PCR, using the primer pair targeted to the coding sequence of GAL1.