FIGURE 4.

Rtt109p stimulates GAL1 transcription. A, Rtt109p promotes the association of RNA polymerase II with the GAL1 core promoter and coding sequence. Both the wild type and RTT109 deletion mutant strains were grown as in Fig. 1B. Immunoprecipitations were performed using 8WG16 antibody (Covance, Inc.) against the C-terminal domain of the Rpb1p subunit of RNA polymerase II. B, RT-PCR analysis. Both the wild type and mutant strains were grown as in A. Total RNA was prepared and analyzed for transcription. Oligo(dT) primer was used for cDNA synthesis. Raf, raffinose.