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The deposition of histones H3 and H2B to GAL1 is significantly increased in the absence of Rtt109p following transcriptional termination. A, histone H3 is deposited more efficiently to the GAL1 core promoter in Δrtt109. Both the wild type and RTT109 deletion mutant strains were initially grown in YPG up to an A600 of 0.9 and then transferred to YPD prior to formaldehyde-based in vivo cross-linking. B, histone H2B is deposited more efficiently to the GAL1 core promoter in Δrtt109.
