FIGURE 1.

Protein sequence alignment of VhACP and other related ACPs. The numbering is performed according to the V. harveyi sequence and begins at Ser-1, which is preceded by a four residue extension present because of the cloning protocol. Anionic residues are highlighted in light gray, and cationic residues are shown in dark gray. The phosphopantetheine attachment site is boxed, and a secondary structure diagram is provided above the sequences. The differences in primary structure between the E. coli and V. harveyi ACP sequences are highlighted in bold. The residues mutated for the calcium-binding site A and B elimination mutants are also shown.