FIGURE 7.

Effects of TH2-3 and COX-2 on intracellular cAMP concentrations. Murine RAW264.7 macrophages or RPM cells were overexpressed (OE) with COX-2-eGFP, and along with the empty vector control, were treated with TH2-3 and LPS under pre-, co-, and post-conditions for 24 h, and the intracellular cAMP from 100 μg total cell protein lysate was measured by an ELISA technique. A, RAW264.7-COX2-OE or RAW264.7 cells at 24 h after treatment. B, RPM-COX-2-OE or RPM cells at 24 h after treatment. Values are the mean of three independent experiments (n = 3). Error bars, S.E. *, values differ at p < 0.05.