FIGURE 8.

Effects of various signaling inhibitors on TH2-3-mediated regulation of proinflammatory cytokines. Overnight-cultured RAW264.7 cells were treated with 10 ng/ml LPS and/or 100 μg/ml TH2-3 in the presence or absence of various phosphatase and kinase inhibitors in serum-free medium for 4 h. Then, secretion of TNF-α in the supernatants of control, LPS, TH2-3, and LPS co-treated with TH2-3 cells was assayed by an ELISA. A, U0126 (a MEK1/2 inhibitor) at 10 μm or 10 μm SB 203508 (a P38/MAPK inhibitor). B, calyculin A (a serine/threonine phosphatase inhibitor) at 10 μm or 10 μm PD98059 (a MEK1 inhibitor). Values are the mean of three independent experiments (n = 3). Error bars, S.E. *, values differ at p < 0.05.