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Effects of TH2-3 on LPS-stimulated pERK expression. RAW264.7 cells were treated with LPS under pre-, co-, and post-conditions for 0.5 h (A) 1 h (B), and 2 h (C). Total cell lysates from three independent experiments were pooled together, and 10 μg of protein from each treatment was subjected to Western blotting against ERK1/2- and pERK1/2-specific primary antibodies followed by an anti-rabbit secondary antibody.
