FIGURE 5.

Binding of purified 1F12 scFv-Fc proteins to LNFPIII-BSA. 1F12 scFv-Fv proteins were purified from media of the NS0 stable cells following the original procedures or the alternative procedures (A and B, respectively) or were purified from media of COS-7 cells transiently transfected with pCI-neo/1F12 scFv-Fc following the alternative procedures (C). Binding of a series of diluted samples of purified 1F12 scFv-Fc proteins was measured by ELISA using LNFPIII-BSA (closed circle) or BSA (open circle) as described under “Experimental Procedures.”