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Induction of BiP, CRT, and EDEM expressions in scFv-Fc protein-producing COS-7 cells measured by RT-PCR. Real time PCR was performed as described under “Experimental Procedures.” Shown are the results using GAPDH mRNA as an endogenous control. The expression levels are normalized with the mock control. Basically, the same results were obtained with the experiments using β-actin as an endogenous control (data not shown).
