FIGURE 1.

2-DE of total A. thaliana Col-0 (WT) root-secreted proteins. Two hundred and fifty micrograms of protein were isoelectrically focused and separated as described under “Experimental Procedures.” A, representative 2-DE gel from root-secreted proteins in WT at day 28 is shown. The molecular masses (kDa) of protein standards are indicated to the left of the gel, and the isoelectric point (pI) is indicated at the top of the gel. The arrows with numbers represent identified proteins that are listed in supplemental Table S1. The gray underlined numbers represent the proteins that are not present in the representative gel but are present in other samples. B, summary of the distribution of root secreted identified protein classes as determined using the protein function data base Pfam and classification schema previously reported for Arabidopsis (91).