FIGURE 4.

MyD88 regulates AA release, eicosanoid production, and COX2 expression. Wild type and MyD88^−/− peritoneal macrophages were stimulated with C. albicans (m.o.i. 5) and 100 μg/ml P-βG for 60 min to measure [³H]AA release (A), and with C. albicans (m.o.i. 2) for 6 h to evaluate COX2 expression on Western blots using antibodies to total ERKs to evaluate sample loading (B), and to measure eicosanoids in the culture medium by mass spectrometry (C). The data are the average of nine experiments for C. albicans and five experiments for P-βG ± S.E. (A), the average of three experiments ± S.E. (C), and a representative of three independent experiments (B). The asterisk in A indicates a significant decrease (p < 0.05) for MyD88^−/− macrophages compared with wild type macrophages. US, unstimulated.