FIGURE 6.

Activation of αvβ3-integrins in β1-k/d cells. A, Surface expression of αvβ3-integrin in β1-k/d or control HKC cells was evaluated by flow cytometry with a monoclonal antibody to αvβ3. no ° Ab indicates without primary antibody. B, β1-k/d or control HKC cells plated on gelatin-coated coverslip were stained following fixation/permeabilization with antibodies to αvβ3-integrin (red) and to phospho-Tyr-397 FAK (green). Signal from Marine blue (blue) was pseudo colored to green by image acquisition software (LSM image analyzer) for better visualization. Colocalization of these molecules was depicted as yellow in the merged images, as well as in the graphical representation of analysis using the CoLocalizer Express software. *, p < 0.05 as compared with control. dpi, dot per inch. C, specific staining with WOW1 antibody that recognizes active αvβ3-integrin was detected with a secondary antibody conjugated with Alexa Fluor 568. Bar = 10 μm. 63×/1.4× oil objective.