FIGURE 2.

Western blotting and densitometric analysis of PMCA2 isoform overexpression in transfected CHO cells. 20 μg of crude membrane proteins from transfected CHO cells, prepared by a freeze and thaw method, were separated by SDS-PAGE as described under “Experimental Procedures” and stained with polyclonal antibody 2N, which recognizes isoform 2 of the pump or against tubulin. The lanes correspond to cells transfected with the indicated variants of PMCAs fused to GFP. The data are representative of at least three independent experiments.