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. 2010 Jul 20;285(40):30837–30850. doi: 10.1074/jbc.M110.128272

FIGURE 7.

FIGURE 7.

Suppression of FOXO3a expression blocks the apoptosis-inducing effect of C/EBPα. A, shown are FOXO3A mRNA levels, assessed by real time Q-PCR, in 4-HT-treated scramble or FOXO3A shRNA-transfected K562 cells (MigRI-transduced or C/EBPα-ER-expressing). HPRT expression was used as internal control. Error bars denote S.D. of normalized means of one representative experiment (of three) performed in triplicate. B, shown is frequency of apoptotic cells (mean plus S.D., three experiments) in scrambled or shRNAFOXO3A-transfected K562 cells (MigRI-transduced or C/EBPα-ER-expressing) after treatment with 0.2 μm IM alone or with 4-HT (250 nm) assessed by ipodiploid percentage after propidium iodide staining. * p < 0.05 relative to corresponding 4-HT-untreated sample.