TABLE 1.
Frequency of undifferentiated and differentiated myeloid cells in 4HT-treated C/EBP ER-transduced K562 cells
Numbers in parentheses are the number of cells.
| C/EBP-ER-transduced K562 cells | Percent differentiated cells |
|||
|---|---|---|---|---|
| Time 0 | 24 h | 48 h | 72 h | |
| % | ||||
| C/EBPα−ER | ||||
| Blasts | 75.7 (318) | |||
| Promyelocytes | 15.7 (66) | 13.9 (36) | ||
| Myelocytes | 8.5 (36) | 30.3 (78) | 12 (25) | |
| Metamyelocytes | 25.6 (66) | 26 (54) | 20.3 (66) | |
| Bands | 21.3 (55) | 37.5 (78) | 45.5 (148) | |
| Segments | 8.9 (23) | 24.5 (51) | 34.2 (111) | |
| Total no. of cells | 100 (420) | 100 (258) | 100 (208) | 100 (325) |
| C/EBPβ−ER | ||||
| Blasts | 73.2 (238) | 14.3 (49) | 14.2 (39) | 34.3 (141) |
| Promyelocytes | 16.6 (54) | 46.5 (159) | 30.4 (90) | 44 (181) |
| Myelocytes | 9.6 (31) | 28.4 (97) | 39 (117) | 12.9 (53) |
| Metamyelocytes | 0.6 (2) | 8.8 (30) | 14 (43) | 8.8 (36) |
| Bands | 2 (7) | 1.7 (5) | ||
| Segments | 0.7 (2) | |||
| Total no. of cells | 100 (325) | 100 (342) | 100 (296) | 100 (411) |
| N-C/EBPα+C/EBPβ-DBD-ER | ||||
| Blasts | 69.2 (275) | 0.3 (1) | ||
| Promyelocytes | 17.9 (71) | 5.3 (15) | 0.3 (1) | 0.9 (2) |
| Myelocytes | 11.9 (47) | 16.4 (47) | 5.5 (16) | 0.4 (1) |
| Metamyelocytes | 1 (4) | 30.6 (88) | 45.3 (132) | 10 (23) |
| Bands | 35.9 (103) | 31.8 (93) | 55.7 (128) | |
| Segments | 11.8 (34) | 16.8 (49) | 33 (76) | |
| Total no. of cells | 100 (397) | 100 (287) | 100 (292) | 100 (230) |
| N-C/EBPβ+C/EBPα-DBD-ER | ||||
| Blasts | 62.4 (164) | 16.8 (55) | 28.8 (79) | 38.4 (119) |
| Promyelocytes | 25.8 (68) | 32.4 (106) | 35 (96) | 46.3 (144) |
| Myelocytes | 10.7 (28) | 34.6 (113) | 26.6 (73) | 9.7 (30) |
| Metamyelocytes | 1.1 (3) | 12.5(41) | 8.4 (23) | 5.6 (17) |
| Bands | 3.7 (12) | 1.2 (3) | ||
| Segments | ||||
| Total no. of cells | 100 (263) | 100 (327) | 100 (274) | 100 (310) |