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Tau constructs employed for transfection of cells. N1 and N2 represent the two amino-terminal inserts, and R1, R2, R3, and R4 represent the four microtubule-binding domain repeats. Indicated are the S/T sites, which were pseudophosphorylated in wild type or R406W mutated Tau (τ) to Glu (E) and as control to Ala (A) in the present study. Asp421 Tau is shown (D421).
