FIGURE 2.

Growth curves and integrity of CdhA-specific mutant and complemented strains. Wild-type (M1-SF370 or M1-WT) and various isogenic mutant strains and corresponding complemented strains were created with different strategies and were grown in THY broth as described under “Experimental Procedures.” A, growth curves of the wild-type (M1-WT) and isogenic mutant strains (M1ΔCdhA mutant lacking CdhA, M1CdhAΔC55 mutant expressing truncated-CdhA, and M1ΔSTK mutant lacking SP-STK (13)); B, corresponding complemented strains (M1ΔCdhA::cdhA and M1ΔSTK::cdhA (SP-STK mutant complemented with cdhA) and CdhA-overexpressing strain (M1-WT::cdhA) were monitored spectrophotometrically by measuring A₆₀₀ (O.D.600 nm) over a period of 18 h. Each data point represents an average of three independent readings. For clarity, error bars have been omitted. Arrows M and L indicate middle and late log phase points for each growth curve. C, Western blot analysis showing the presence and absence of CdhA expression in the whole lysates of mutant, wild-type, and complemented strains using anti-CdhA IgG (lane 1), recombinant CdhA protein, and whole cell lysates of wild type M1-SF370 (lane 2), M1ΔCdhA (lane 3), M1CdhAΔC55 (lane 4), M1ΔCdhA::cdhA (lane 5), M1-WT::cdhA (lane 6), M1ΔSTK (lane 7), and M1ΔSTK::cdhA (lane 8).