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Comparison of human immunoglobulin binding property of CdhA mutants with that of the wild-type GAS strain. Ligand-binding assays were performed in 96-well formats, as described under “Experimental Procedures.” Each data point represents an average ± S.E. (error bars) of three individual experiments performed in triplicates. Final readings represent values obtained after subtracting the average value obtained from 12 control wells.
