TABLE 3.
Effect of HIV-1 Nef on the diffusion rates of HLA-I A2 and LL/AA CD4 at the PM deduced by FRAP
Effect of HIV-1 Nef on the diffusion rates of HLA-I A2 in different subcellular organelles deduced from the FRAP assay. Dt,fast or Dt,slow and % fast or slow represent diffusion rates of the fast or slow components and their relative abundance (in %).
| A2-Cer and A2-Venus | Nef-Cer and A2-Venus | LL/AA CD4-Cer and LL/AA CD4-Venus | Nef-CerFP and LL/AA CD4-Venus | |
|---|---|---|---|---|
| t½ (s) | 0.50 | 1.09 | 2.09 | 1.60 |
| tfast | 0.23 ± 0.04 | 0.27 ± 0.11 | 0.86 ± 0.25 | 0.68 ± 0.35 |
| Dfast (μm2/s) | 0.27 | 0.23 | 0.07 | 0.09 |
| % fast | 40 ± 3 | 25 ± 4 | 32 ± 5 | 27 ± 7 |
| tslow | 4.1 ± 0.5 | 4.5 ± 0.7 | 8.6 ± 4.2 | 5.0 ± 2.0 |
| Dslow (μm2/s) | 0.02 | 0.01 | 0.01 | 0.01 |
| % slow | 40 ± 2 | 43 ± 2 | 50 ± 4 | 48 ± 9 |
| % immobile | 20 ± 1 | 31 ± 2 | 19 ± 8 | 25 ± 3 |