TABLE 4.
Summary of results for the molecular radii calculated from the respective diffusion coefficients using the Saffman and Delbruck Equation 5 (D <2 μm2/s)
The Stokes Einstein equation was applied for coefficients above this value using a η = 6 centipoise.
| Protein(s) at the PM with fast/slow component | Diffusion coefficients | Radius (nm) | Fraction (%) |
|---|---|---|---|
| A2-eYFP | |||
| Fast | 6.0 | 5.96 | 50 |
| Slow | 0.21 | 39.8 | 50 |
| A2-Venus + Nef-CerFP | |||
| Fast | 4.9 | 7.29 | 60 |
| Slow | 0.23 | 38.2 | 40 |
| W632–647 + Nef-CerFP | 0.72 | 14 | 100 |
| B7.2–647 + Nef-CerFP | 0.39 | 28 | 100 |
| MβCD treatment | |||
| A2-Venus + Nef-CerFP | |||
| Fast | 1.76 | 2 | 54 |
| Slow | 0.46 | 24 | 46 |
| IKA treatment | |||
| A2-Venus + Nef-CerFP | |||
| Fast | 2.3 | 15 | 72 |
| Slow | 0.21 | 40 | 28 |
| LL/AA CD4-Venus only | 0.9 | 10 | 100 |
| LL/AA CD4-Venus + Nef-CerFP | |||
| Fast | 6.6 | 5.42 | 55 |
| Slow | 0.32 | 32 | 45 |
| WT CD4-Venus + Nef-CerFP | |||
| Fast | 7.8 | 4 | 31 |
| Slow | 0.37 | 29 | 69 |
| MβCD treatment | |||
| LL/AA CD4-Venus + Nef-CerFP | |||
| Fast | 3.7 | 10 | 50 |
| Slow | 0.21 | 40 | 50 |
| IKA treatment | |||
| LL/AA CD4-eYFP + Nef-CerFP | |||
| Fast | 2.3 | 15 | 68 |
| Slow | 0.09 | 51 | 32 |