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. 2010 Jul 9;285(40):30884–30905. doi: 10.1074/jbc.M110.135947

TABLE 4.

Summary of results for the molecular radii calculated from the respective diffusion coefficients using the Saffman and Delbruck Equation 5 (D <2 μm2/s)

The Stokes Einstein equation was applied for coefficients above this value using a η = 6 centipoise.

Protein(s) at the PM with fast/slow component Diffusion coefficients Radius (nm) Fraction (%)
A2-eYFP
    Fast 6.0 5.96 50
    Slow 0.21 39.8 50

A2-Venus + Nef-CerFP
    Fast 4.9 7.29 60
    Slow 0.23 38.2 40
    W632–647 + Nef-CerFP 0.72 14 100
    B7.2–647 + Nef-CerFP 0.39 28 100

MβCD treatment
    A2-Venus + Nef-CerFP
    Fast 1.76 2 54
    Slow 0.46 24 46

IKA treatment
    A2-Venus + Nef-CerFP
        Fast 2.3 15 72
        Slow 0.21 40 28
    LL/AA CD4-Venus only 0.9 10 100
    LL/AA CD4-Venus + Nef-CerFP
        Fast 6.6 5.42 55
        Slow 0.32 32 45
    WT CD4-Venus + Nef-CerFP
        Fast 7.8 4 31
        Slow 0.37 29 69

MβCD treatment
    LL/AA CD4-Venus + Nef-CerFP
        Fast 3.7 10 50
        Slow 0.21 40 50

IKA treatment
    LL/AA CD4-eYFP + Nef-CerFP
    Fast 2.3 15 68
    Slow 0.09 51 32