TABLE 5.

FRAP analysis of diffusion rates of HLA-I-A2 at the plasma membrane in the context Nef expression after cholesterol depletion

	PM A2C-A2V	PM NA7C-A2V	MβCD/PM A2C and A2V	MβCD/PM NA7C and A2V
t½ (s)	0.5	1.1	1	1.6
t(fast)	0.23 ± 0.04	0.27 ± 0.11	0.07	0.167
Dffast (μm2/s)	0.27	0.23	0.90	0.38
% fast	40 ± 3	25 ± 4	19	25
t(slow)	4 ± 0.5	4 ± 0.7	3	6.3
Dfslow (μm2/s)	0.015	0.014	0.022	0.01
% slow	40 ± 2	43 ± 2	45	46
% immobile	20 ± 1	31 ± 2	36	29