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. 2010 Jun 21;285(40):31002–31010. doi: 10.1074/jbc.M110.136085

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© 2010 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 2. — ES derivation efficiency from SCNT embryos were increased with CBHA treatment. A, improved efficiency of NT-ES cell derivation from CBHA- and TSA-treated SCNT blastocysts is shown. B, expansion of the outgrowths formed by non-treated (0) and CBHA-treated (20 μm) SCNT blastocysts is shown. Area of each outgrowth was measured at days 2, 3, and 4 after plating of the blastocysts. C, shown is expression of Oct4 in day 4 outgrowths derived from NT blastocysts derived from non-treated (0 μm) embryos or CBHA treated ones at 20 μm.