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NT-ES cells derived from CBHA treated embryos exhibit normal features. A, correct expression of Oct4, Sox2, and SSEA1 is shown. B–D, teratomas from mouse NT-ES cells show differentiated derivatives of all three embryonic germ layers: B, minor salivary gland (endoderm); C, cartilage tissue (mesoderm); D, neural tube (ectoderm). E, positive alkaline phosphatase activity is shown. F, shown is a chimera mouse born from CBHA NT-ES.
