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. 2010 Jul 27;285(40):31073–31086. doi: 10.1074/jbc.M110.154484

FIGURE 1.

FIGURE 1.

SPRET/Ei mice are very resistant to LPS lethality and show impaired basal and LPS-induced gene expression. Survival (A) and serum IL-6 levels in C57BL/6 and SPRET/Ei mice after intraperitoneal injection of 500 μg of LPS (B) are shown. Mortality was monitored for 60 h (no further deaths occurred). Black bars, C57BL/6 mice (n = 6); white bars, SPRET/Ei mice (n = 4). C, LPS-induced gene expression in liver shows reduced levels of GR-transrepressed genes in SPRET/Ei mice compared with control C57BL/6 mice. C57BL/6 (n = 4, black bars) and SPRET/Ei mice (n = 4, white bars) were injected with 500 μg of LPS intraperitoneally, and mRNA was isolated 0 and 6 h after injection. qPCR was used to measure mRNA levels of a set of GR-repressed genes encoding IL-6, IL-1β, IFNγ, TNF, CCL5, and CXCL10. D, shown are IFNβ mRNA (left) and protein (right) levels in C57BL/6 (n = 3, black bars) and SPRET/Ei (n = 3, white bars) mice at different time points after LPS (500 μg) stimulation. ND, not detectable. Significance was calculated for difference between C57BL/6 and SPRET/Ei.