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. Author manuscript; available in PMC: 2010 Sep 25.
Published in final edited form as: Nat Immunol. 2009 Feb 1;10(3):314–324. doi: 10.1038/ni.1698

Figure 4. Full differentiation of effector TH-17 cells requires IL-23R in vivo.

Figure 4

(a–e) Analysis of phenotype of wild-type or Il23ra−/− CD45.1+CD4+OTII T cells after transfer into wild-type recipients and immunization with OVA(323–339). (a) Flow cytometry for cell surface expression of CD44, CCR7 and CD62L on wild-type and Il23ra−/− OTII T cells (shaded histograms) overlaid with total CD4+ cells for comparison (clear histograms) at day 7 post-immunization. b) Surface IL-7Rα expression on wild-type (shaded histogram) and Il23ra−/− (clear histogram) OTII cells from dLN. c) RT-PCR analysis of IL-7Rα expression in OTII+ cells sorted by flow cytometry from dLN; values are normalized to ubiquitin expression. As a control, total OTII–CD4+ cells were isolated at the same time from recipients of the indicated OTII cells. Data shown are mean ± s.d. of two groups of samples each containing 5 mice per condition. d) Surface CD27 expression on OTII cells in dLN and blood. e) Frequency of wild-type and Il23ra−/− OTII cells (as proportion of total CD4+ cells) in blood at indicated time points post immunization. Data shown are representative of 3 experiments with similar results.