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. Author manuscript; available in PMC: 2011 Sep 24.
Published in final edited form as: Mol Cell. 2010 Sep 24;39(6):975–987. doi: 10.1016/j.molcel.2010.08.027

Figure 2. FzlA and FzlC are uncharacterized FtsZ binding proteins.

Figure 2

A) Localization of mCherry fusions to class I candidate proteins in cells overexpressing ftsZ*. Fluorescence (red) overlaid on PC (top) and fluorescence (bottom) are shown. Scale bar = 2 μm. B) Localization of mCherry fusions to class II proteins in cells overexpressing ftsZ*. See also Fig. S1. C) Co-localization of mCherry fusions to FzlA and FzlC (red) with FtsZ-CFP (green) in wild type cells. In the merged image, fluorescence is overlaid on phase contrast. Scale bar = 2 μm. D) Localization of mCherry fusions to FzlA and FzlC in cells depleted of FtsZ (-FtsZ) and after FtsZ depletion followed by repletion of FtsZ for 1 h (FtsZ repletion). Fluorescence and PC images are shown. Scale bar = 2 μm. E) Coomassie-stained SDS PAGE of supernatant (right) and pellet (left) from co-sedimentation reactions containing 3 μM of the candidate protein ± 3 μM FtsZ.