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. Author manuscript; available in PMC: 2011 Sep 24.
Published in final edited form as: Mol Cell. 2010 Sep 24;39(6):975–987. doi: 10.1016/j.molcel.2010.08.027

Figure 3. FzlA is critical for cell division and is cell cycle regulated.

Figure 3

A) Genomic context of fzlA (WT) and construction of a xylose-dependent FzlA depletion strain (EG312). B) Immunoblots of cell lysates from wild type (WT) cells or cells from strain EG312 grown in the absence of xylose for the indicated times (hours). Equivalent total OD units of lysates were probed with antibodies recognizing the indicated proteins. C) Phase contrast images of cells of strain EG312 grown in the absence of xylose (FzlA depleted) for the indicated times. Scale bar = 2 μm. D) PC and mCherry-FzlA fluorescence images showing FzlA localization over the cell cycle. Synchronized swarmer cells were placed on M2G-agarose pads and imaged every 30 min. E) Normalized abundance of ftsZ and fzlA transcript levels over the cell cycle. F) Immunoblots using antibodies against the indicated proteins of cell lysates from synchronized wild type cells grown in M2G (top) or PYE (bottom). See also Fig. S2.