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Preferential insertion of the individual dNTPs opposite a THF abasic site located on the 5′-terminus (A-27) of an A4 tract by T7 Sequenase. The dNTP concentrations used were 0.005, 0.5, 5, 50 and 500 μM (for dATP the 500 μM concentration is not shown). Lanes with no added dNTP and with all dNTPs are included. dATP and dGTP are most efficiently inserted opposite the THF lesion.
