Table 1.

Sequences of oligomers and primers used in the studies.

Oligomer name	Sequence	Length	Modification
Primers
P20	5′ CCG TAG GAC TGC CGT AGT GC 3′	20 nt	5′-Cy5
P23	5′ CCG TAG GAC TGC CGT AGT GCT GG 3′	23 nt	5′-Cy5
P24	5′ CCG TAG GAC TGC CGT AGT GCT GGT 3′	24 nt	5′-Cy5
P25	5′ CCG TAG GAC TGC CGT AGT GCT GGT T 3′	25 nt	5′-Cy5
P26	5′ CCG TAG GAC TGC CGT AGT GCT GGT TT 3′	26 nt	5′-Cy5
P27	5′ CCG TAG GAC TGC CGT AGT GCT GGT TTN 3′ (N = C, G, T or A)	27 nt	5′-Cy5
Templates
Unmodified	5′ ATC CAA CCT GTA TCA TAC TTA CCA AAA CCA GCA CTA CGG CAG TCC TAC GG 3′	50 nt	—
3-m-c3A/24	5′ ATC CAA CCT GTA TCA TAC TTA CCA AAXCCA GCA CTA CGG CAG TCC TAC GG 3′	50 nt	X = 3-m-c3A
3-m-c3A/25	5′ ATC CAA CCT GTA TCA TAC TTA CCA AXA CCA GCA CTA CGG CAG TCC TAC GG 3′	50 nt	X = 3-m-c3A
3-m-c3A/26	5′ ATC CAA CCT GTA TCA TAC TTA CCA XAA CCA GCA CTA CGG CAG TCC TAC GG 3′	50 nt	X = 3-m-c3A
3-m-c3A/27	5′ ATC CAA CCT GTA TCA TAC TTA CCX AAA CCA GCA CTA CGG CAG TCC TAC GG 3′	50 nt	X = 3-m-c3A
c3A/24	5′ ATC CAA CCT GTA TCA TAC TTA CCA AAX CCA GCA CTA CGG CAG TCC TAC GG 3′	50 nt	X = c3A
c3A/25	5′ ATC CAA CCT GTA TCA TAC TTA CCA AXA CCA GCA CTA CGG CAG TCC TAC GG 3′	50 nt	X = c3A
c3A/26	5′ ATC CAA CCT GTA TCA TAC TTA CCA XAA CCA GCA CTA CGG CAG TCC TAC GG 3′	50 nt	X = c3A
c3A /27	5′ ATC CAA CCT GTA TCA TAC TTA CCX AAA CCA GCA CTA CGG CAG TCC TAC GG 3′	50 nt	X = c3A
THF/24	5′ ATC CAA CCT GTA TCA TAC TTA CCA AAX CCA GCA CTA CGG CAG TCC TAC GG 3′	50 nt	X = THF
THF/25	5′ ATC CAA CCT GTA TCA TAC TTA CCA AXA CCA GCA CTA CGG CAG TCC TAC GG 3′	50 nt	X = THF
THF/26	5′ ATC CAA CCT GTA TCA TAC TTA CCA XAA CCA GCA CTA CGG CAG TCC TAC GG 3′	50 nt	X = THF
THF/27	5′ ATC CAA CCT GTA TCA TAC TTA CCX AAA CCA GCA CTA CGG CAG TCC TAC GG 3′	50 nt	X = THF
DFO	5′-TGGTTTTGGT	10 nt