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Activity of ApPolη in organic solvent-containing solutions. (a) Incorporation of dCMP in the presence of DMSO, ethanol, and IPA at a concentration of 20% (v/v) was analyzed, using the primer-template shown in Figure 7. Replication assays were performed at 30°C for 10 minutes. (b) The same assays were performed with various concentrations of DMSO.
