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. Author manuscript; available in PMC: 2011 Apr 1.
Published in final edited form as: Mol Microbiol. 2010 Feb 28;76(2):365–377. doi: 10.1111/j.1365-2958.2010.07099.x

Fig. 1.

Fig. 1

Deletion of the NAD+ de novo pathway genes, nadABC, in M. bovis and M. tuberculosis. A. NAD+ biosynthetic and salvage pathways. NadA, NadB, and NadC enzymes are unique to the NAD+ de novo biosynthesis pathway, while the two last enzymes, NadD and NadE, are common to both the de novo and the salvage NAD+ pathways. The salvage pathway decomposes NAD+ into nicotinamide (NAm) or NMN, or converts NAm from the media to nicotinic acid mononucleotide (NAMN) using 2 enzymes, PncA and PncB. The enzymes and intermediates involved in this study are indicated in bold. B.Southern analysis of M. bovis ΔnadABC and M. tuberculosis ΔnadABC transductants. The diagram shows the replacement of the nadABC operon in M. bovis by a hygromycin resistance gene (hyg) using specialized transduction. Southern analysis of the genomic DNA from wt and ΔnadABC mutants, digested with BamH1, confirmed the replacement of the nadABC genes by hyg in M. bovis and M. tuberculosis.