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. Author manuscript; available in PMC: 2011 Apr 1.
Published in final edited form as: Mol Microbiol. 2010 Feb 28;76(2):365–377. doi: 10.1111/j.1365-2958.2010.07099.x

Fig. 4.

Fig. 4

Growth of M. bovis ΔnadABC pMV261::pncATB in vitro. M. bovis ΔnadABC was transformed with M. tuberculosis pncA cloned into the replicative plasmid pMV261. A. M. bovis ΔnadABC pMV261::pncATB and its parent strain M. bovis ΔnadABC were grown in NAm-containing media, washed 5 X in PBS, diluted, and inoculated in media containing NAm (20 and 0.1 mg/l). Growth was followed by measuring OD600nm at diverse time points. B. M. bovis ΔnadABC pMV261::pncATB and M. bovis ΔnadABC were grown and washed as described above, diluted 1-50, and inoculated in media without NAm (20 mg/l). At each time, samples were taken, diluted, and plated onto Middlebrook 7H10 plates containing NAm (20 mg/l). The plates were incubated at 37°C for 4-5 weeks and colonies were counted.